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KMID : 1023120140120060939
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Korean Journal of Aesthetic Society
2014 Volume.12 No. 6 p.939 ~ p.944
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Mitogen-Activated Protein Kinases Mediated Regulation of Matrix-Metalloprotease 1 by Nobiletin in Human Dermal Fibroblasts
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Cha Hwa-Jun
Kim Young-Joo
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Abstract
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Nobiletin is widely used for the treatment of cancer and inflammation disorder. In neurocytes and melanocytes, nobiletin exposure induced dendrite formation. Recently, nobiletin is suggested as a new whitening agent. However, in dermis, effects of nobiletin are not understood currently. Therefore, it is important to determine how nobiletin exerts its therapeutic action and its effect on the anti-wrinkle. To determine whether MMP1 gene expression is regulated by nobiletin, we measured MMP1 mRNA expression in nobiletein-treated human dermal fibroblasts (HDFs). And we showed that transactivation activity of TPA responsive element (TRE) which is a major regulation promoter element and mitogen-activated protein kinase (MAPK) activity which main regulator of AP-1 complex. As shown results, nobiletin was decrease expression level of MMP1 mRNA in oxidative-stressed HDFs. In addition, nobiletin repressed MAPK phosphorylation and transcriptional activity of AP-1 complex in oxidative-stressed HDFs. Therefore, nobiletin protects oxidative-stress through repressing MAPK phosphorylation and transcriptional activity of AP-1 complex. In addition, these results suggest that nobiletin is a potential cosmetic ingredient repressed antiaging and anti-wrinkle in skin.
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KEYWORD
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Nobiletin, Human dermal fibroblasts, Matrix-metalloprotease 1
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